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Lambic Digest V1 #059
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lambic-digest Tuesday, 5 May 1998 Volume 01 : Number 059
cycloheximide
sterility
so. cal. festival
----------------------------------------------------------------------
From: Jeremy Bergsman <jeremybb at leland.Stanford.EDU>
Date: Mon, 04 May 1998 16:41:04 -0700
Subject: cycloheximide
Cycloheximide can be autoclaved, or it can simply be made up in ethanol
and you can call that "sterile".
- --
Jeremy Bergsman
jeremybb at stanford.edu
http://www-leland.stanford.edu/~jeremybb
------------------------------
From: Jim Liddil <jliddil at azcc.arizona.edu>
Date: Mon, 04 May 1998 17:01:13 -0700
Subject: sterility
>After reading George Fix's description for making up tubes of HLP (Hsu's
>Lactobacillus and Pediococcus) media in *Analysis of Brewing Techniques*, I
>got to wondering whether the powdered variety can be autoclaved. I gather
>that the cyclohexamide breaks down with heat, but to what degree? Without
>autoclaving, how can one be assured of sterility?
You can autoclave it. The better methods is to add steriel filtered
solution to the melted media and gently mix it. You can also dissolve
cycloheximide in 70% ethanol to make a 10 mg/ml stock solution which will
be close enough to sterile for brewing needs.
Jim
------------------------------
From: B/S Sjoberg <sjoberg at cts.com>
Date: Mon, 4 May 1998 20:55:44 -0800
Subject: so. cal. festival
Last weekend was the Southern California
homebrewer's festival at Lake Skinner -
for anyone interested there were some AWESOME
lambics there..........if they were yours, thanks for
bringing them.
------------------------------
End of lambic-digest V1 #59
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