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dictyNews Volume 39 Number 17
dictyNews
Electronic Edition
Volume 39, number 17
June 14, 2013
Please submit abstracts of your papers as soon as they have been
accepted for publication by sending them to dicty@northwestern.edu
or by using the form at
http://dictybase.org/db/cgi-bin/dictyBase/abstract_submit.
Back issues of dictyNews, the Dicty Reference database and other
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Abstracts
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ABC transporters in Dictyostelium discoideum development
E.R. Miranda, O. Zhuchenko, M. Toplak, B. Santhanam, B. Zupan,
A. Kuspa and G. Shaulsky
Baylor College of Medicine and University of Ljubljana
PLoS One, Accepted
ATP-binding cassette (ABC) transporters can translocate a broad
spectrum of molecules across the cell membrane including physiological
cargo and toxins. ABC transporters are known for the role they play in
resistance towards anticancer agents in chemotherapy of cancer patients.
There are 68 ABC transporters annotated in the genome of the social
amoeba Dictyostelium discoideum. We have characterized more than
half of these ABC transporters through a systematic study of mutations in
their genes. We have analyzed morphological and transcriptional
phenotypes for these mutants during growth and development and found
that most of the mutants exhibited rather subtle phenotypes. A few of the
genes may share physiological functions, as reflected in their transcriptional
phenotypes. Since most of the abc transporter mutants showed subtle
morphological phenotypes, we utilized these transcriptional phenotypes to
identify genes that are important for development by looking for transcripts
whose abundance was unperturbed in most of the mutants. We found a
set of 668 genes that includes many validated D. discoideum developmental
genes. We have also found that abcG6 and abcG18 may have potential
roles in intercellular signaling during terminal differentiation of spores and
stalks.
Submitted by Gad Shaulsky [gadi@bcm.edu]
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[End dictyNews, volume 39, number 17]
