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dictyNews Volume 31 Number 16
dictyNews
Electronic Edition
Volume 31, number 16
November 28, 2008
Please submit abstracts of your papers as soon as they have been
accepted for publication by sending them to dicty@northwestern.edu
or by using the form at
http://dictybase.org/db/cgi-bin/dictyBase/abstract_submit.
Back issues of dictyNews, the Dicty Reference database and other
useful information is available at dictyBase - http://dictybase.org.
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Abstracts
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Dictyostelium Tom1 participates to an ancestral ESCRT-0 complex
Cédric Blanc, Steve J Charette, Sara Mattei, Laurence Aubry, Ewan W. Smith,
Pierre Cosson and François Letourneur
Traffic, in press
Sorting of ubiquitinated proteins to multivesicular bodies (MVBs) in mammalian
cells relies on proteins with a VHS (Vps27/Hrs/STAM) domain. Here, we show that
the amoeba Dictyostelium presents only one protein with a VHS domain: DdTom1.
We demonstrate that the VHS domain of DdTom1 is followed by a GAT (GGA and
Tom1) domain which binds ubiquitin, and by a non conserved C-terminal domain
which can recruit clathrin, Eps15 (EGFr pathway substrate 15) and Tsg101 (tumor
susceptibility gene 101), a component of the MVB biogenesis machinery (ESCRT
complexes). Both VHS and GAT domains interact with phospholipids and therefore
could ensure the recruitment of DdTom1 to endosomal membranes. We propose
that DdTom1 participates in an ancestral ESCRT-0 complex implicated in the
sorting of ubiquitinated proteins into MVBs.
Submitted by: François Letourneur [francois.letourneur@ibcp.fr]
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[End dictyNews, volume 31, number 16]
