dictyNews Volume 27 Number 10

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Dicty News

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dictyNews 
Electronic Edition 
Volume 27, number 10 
September 29, 2006 

Please submit abstracts of your papers as soon as they have been 
accepted for publication by sending them to dicty@northwestern.edu 
or by using the form at 
http://dictybase.org/db/cgi-bin/dictyBase/abstract_submit. 

Back issues of dictyNews, the Dicty Reference database and other  
useful information is available at dictyBase - http://dictybase.org. 

 
=============== 
  Announcement 
=============== 

 
Dear colleagues, 

The "Dictyostelium discoideum Protocols" book which was recently published 
in the "Methods in Molecular Biology" series from Humana Press can now be 
ordered from the Humana website (http://www.humanapress.com/ ; search for 
Dictyostelium). The price is $130,50. 

Best wishes 

Ludwig Eichinger [ludwig.eichinger@uni-koeln.de] 
Francisco Rivero [francisco.rivero@uni-koeln.de] 

 

============= 
  Abstracts 
============= 

 

Necrotic cell death in a protist 

C Laporte1,2,3, A Kosta1,2,3, G Klein4, L Aubry4, D Lam1,2,3, E Tresse1,2,3, 
MF Luciani1,2,3 and P Golstein*,1,2,3 

1Centre d'Immunologie de Marseille-Luminy, UniversitŽ de la MŽditerranŽe,  
Case 906, 13288 Marseille Cedex 9, France;  
2INSERM U631, 13288 Marseille cedex 9, France;  
3CNRS UMR6102, 13288 Marseille cedex 9, France;  
4CEA-Grenoble / DRDC / BBSI, UMR 5092 CNRS-CEA-UJF, 17, rue des Martyrs,  
38054 Grenoble Cedex 09, France 

 
Cell Death and Differentiation, in press. Advanced online publication.  
doi: 10.1038/sj.cdd.4401994. 

While necrotic cell death is attracting considerable interest, its molecular  
bases are still poorly understood. Investigations in simple biological  
models, taken for instance outside the animal kingdom, may benefit from less  
interference from other cell death mechanisms and from better experimental  
accessibility, while providing phylogenetic information. Can necrotic cell  
death occur outside the animal kingdom ? In the protist Dictyostelium,  
developmental stimuli induced in an autophagy mutant a stereotyped sequence  
of events characteristic of necrotic cell death. This sequence included swift  
mitochondrial uncoupling with mitochondrial DC-FDA fluorescence, ATP  
depletion and increased oxygen consumption. This was followed by perinuclear  
clustering of dilated mitochondria. Rapid plasma membrane rupture then  
occurred, which was evidenced by time-lapse videos and quantified by FACS.  
Of additional interest, developmental stimuli and classical mitochondrial  
uncouplers triggered a similar sequence of events, and exogenous glucose  
delayed plasma membrane rupture in a non-glycolytic manner. The occurrence  
of necrotic cell death in the protist Dictyostelium (1) provides a very  
favorable model for further study of this type of cell death, and (2)  
strongly suggests that the mechanism underlying necrotic cell death was  
present in an ancestor common to the Amoebozoa protists and to animals and  
has been conserved in evolution. 

 
Submitted by: Pierre Golstein [golstein@ciml.univ-mrs.fr] 
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[End dictyNews, volume 27, number 10]