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dictyNews Volume 28 Number 04
dictyNews
Electronic Edition
Volume 28, number 4
February 9, 2007
Please submit abstracts of your papers as soon as they have been
accepted for publication by sending them to dicty@northwestern.edu
or by using the form at
http://dictybase.org/db/cgi-bin/dictyBase/abstract_submit.
Back issues of dictyNews, the Dicty Reference database and other
useful information is available at dictyBase - http://dictybase.org.
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Abstracts
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A stress response kinase, KrsA, controls cAMP relay during the early
development of Dictyostelium discoideum
Tetsuya Muramoto#, Hidekazu Kuwayama#, Kumiko Kobayashi,
and Hideko Urushihara
# These two authors are equally contributed.
Developmental Biology, in press
Solitary amoebae of Dictyostelium discoideum are frequently exposed to
stressful conditions in nature, and their multicellular development is
one response to environmental stress. Here we analyzed an aggregation
stage abundant gene, krsA, homologous to human krs1 (kinase responsive to
stress 1) to understand the mechanisms for the initiation of development
and cell fate determination. The krsA- cells exhibited reduced viability
under hyperosmotic conditions. They produced smaller aggregates on membrane
filters and did not form aggregation streams on a plastic surface under
submerged starvation conditions, but were normal in sexual development.
During early asexual development, the expression of cAMP-related genes
peaked earlier in the knockout mutants. Neither cAMP oscillation in starved
cells nor an increase in the cAMP level following osmotic stress was
observed in krsA-. The nuclear export signal, as well as the kinase domain,
in KrsA was necessary for stream formation. These results strongly suggest
that krsA is involved in cAMP relay, and that signaling pathways for
multicellular development have evolved in unison with the stress response.
Submitted by Hideko Urushihara [hideko@biol.tsukuba.ac.jp]
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[End dictyNews, volume 28, number 4]
