dictyNews Volume 26 Number 14

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dictyNews 
Electronic Edition 
Volume 26, number 14 
May 12, 2006 

Please submit abstracts of your papers as soon as they have been 
accepted for publication by sending them to dicty@northwestern.edu 
or by using the form at 
http://dictybase.org/db/cgi-bin/dictyBase/abstract_submit. 

Back issues of dictyNews, the Dicty Reference database and other  
useful information is available at dictyBase - http://dictybase.org. 

 
============= 
  Abstracts 
============= 

 

Title: Clathrin Light Chain: Importance of the Conserved Carboxy-Terminal  
Domain to Function in Living Cells 
Authors:  Jingshan Wang, Yanqin Wang, and Theresa J. O'Halloran 

 
Traffic, in press 

Clathrin triskelions assemble into coats capable of packaging  
membrane and receptors for transport to intracellular destinations. A  
triskelion is formed from three heavy chains bound to three light chains.  
All clathrin light chains contain an acidic amino terminal domain, a central  
coiled segment and a carboxy-terminal domain conserved in amino acid  
sequence. To assess their functional contribution in vivo, we expressed  
tagged segments of the Dictyostelium clcA in clathrin light chain-minus  
Dictyostelium (clc null) cells. We examined the ability of these clcA  
fragments to rescue clathrin phenotypic deficiencies, to cluster into  
punctae on membranes, and to bind to the heavy chain. When expressed in  
clc null cells, a clcA fragment containing the amino-terminal domain and  
the central coiled domain bound heavy chain, but was dispensable for  
clathrin function.  Instead, the carboxy-terminal domain of clcA was a  
critical determinant for association with punctae, for clathrin function  
and for robust binding to the heavy chain. A 70 amino acid carboxy-terminal  
fragment was necessary and sufficient for full function, and for  
localization into punctae on intracellular membranes. A shorter 49 amino  
acid carboxy-terminal fragment could distribute into punctae, but failed  
to rescue developmental deficiencies. These results reveal the importance  
of the carboxy-terminal domain of the light chain in vivo. 

 
Submitted by: Terry OÕHalloran [t.ohalloran@mail.utexas.edu] 
----------------------------------------------------------------------------- 

 
============== 
 Announcement 
============== 

 
A special issue of European Journal of Cell Biology (Vol. 85, issue 8/9) is  
being published to honour Guenther Gerisch on the occasion of his 75.  
birthday (which was on May 2). The issue contains research articles and  
reviews, which have been compiled for this occasion by former students and  
colleagues who have collaborated with Guenther in the past.  

While we wish to Guenther all the best on the occasion of his birthday, we  
want to acknowledge with this initiative Guenther's influential contributions  
to cell biology in 50 years research, which were also groundbreaking for  
establishing Dictyostelium as a leading model organism. 

The content of the issue is the following:  

Editorial 

* Annette Mueller-Taubenberger and Salvatore Bozzaro: From cell-cell adhesion  
and cellular oscillations to spectacular views inside the cell - 50 years of  
research with Dictyostelium  

* John Heuser: Evidence for recycling of contractile vacuole membrane during  
osmoregulation in Dictyostelium amoebae 

Reviews 

* Atsuo T. Sasaki, Richard A. Firtel: Regulation of chemotaxis by the  
orchestrated activation of Ras, PI3K, and TOR 

* Tian Jin, Dale Hereld: Moving toward understanding eukaryotic chemotaxis 

* Igor Weber: Is there a pilot in a pseudopod?    

* Sara Mattei, GŽrard Klein, Michel Satre, Laurence Aubry: Trafficking and  
developmental signalling: Alix at the crossroads    

* Yogikala Prabhu, Ludwig Eichinger: The Dictyostelium repertoire of seven  
transmembrane receptors     

* Georgia Vlahou, Francisco Rivero: Rho GTPase signaling in Dictyostelium  
discoideum: insights from the genome 

* Hideko Urushihara, Tetsuya Muramoto: Sexual reproduction and its genetic  
control in Dictyostelium 

* Leonard Bosgraaf, Peter J. M. van Haastert: Regulation of myosin II in  
Dictyostelium 

Original articles 

* Song, L., Nadkarni, S., Bodeker, H., Beta, C., Bae, A., Frank, C.,  
Rappel, W-J., Loomis, W. F. and Bodenschatz, E.: Dictyostelium discoideum  
chemotaxis: threshold for directed motion 

* Dirk Wienke, Anja Drengk, Nicole Jenne, Markus Maniak: Vacuolin, a  
flotillin/reggie-like protein from Dictyostelium oligomerizes for endosomes  
association 

* Margaret Clarke, Lucinda Maddera: Phagocyte meets Prey: Uptake,  
internalization, and killing of bacteria by Dictyostelium amoebae   

* Marija Marko, Yogikala Prabhu, Rolf Mueller, Rosemarie Blau-Wasser, Michael  
Schleicher, Angelika A. Noegel: The annexins of Dictyostelium  

* Daniel Rosel, Alan R. Kimmel: The COP9 signalosome regulates cell  
profileration of Dictyostelium discoideum   

* Axel Strehle, Michael Schleicher and Hans Faix: Trix, a novel Rac guanine  
nucleotide exchange factor in Dictyostelium discoideum is associated with  
the plasma membrane and late endosomes   

* Katrina Boeckeler, Kathryn Adley, Xuehua Xu, Alex Jenkins, Tian Jin,  
Robin S. B.  Williams: The neuroprotective agent, valproic acid, regulates  
the mitogen-activated protein kinase pathway through modulation of  
protein A signalling in Dictyostelium discoideum     

* Rajesh Arasada, Hyunju Son, Nagendran Ramalingam, Ludwig Eichinger,  
Michael Schleicher, Meino Rohlfs: Characterization of the STE20 like kinase  
Krs1 of Dictyostelium discoideum   

* Alessandra Balbo, Salvatore Bozzaro: Cloning of Dictyostelium eIF6  
(p27BBP) and mapping its nucle(ol)ar localization subdomains  

* Katrin V. Koch, Yvonne Reinders, Thi-Hieu Ho, Albert Sickmann, Ralph Graef: 
Identification and isolation of Dictyostelium microtubule-associated protein  
interactors by tandem affinity purification  

* Alice Y. Pollitt, Simone L. Blagg, Neysi Ibarra, Robert H. Insall: Cell  
motility and Scar localisation in axenically growing Dictyostelium cells  
(Short communication) 

* Esther Bandala-Sanchez, Sarah J. Annesley, Paul Fisher: A phototaxis  
signalling complex in Dictyostelium discoideum (Short communication)   

* Angelika Boettger, Dominik Strasser, Margherita Lasi, Silvia Fischer,  
Alice Levine, Olga Alexandrova and Charles N. David: Genetic screen for  
signal peptides in Hydra reveals a novel class of sequences supporting  
protein secretion    

* Annette Mueller-Taubenberger, Michel Vos, Angelika Boettger, Margherita Lasi, 
Frank P. L. Lai, Markus Fischer, Klemens Rottner: Monomeric red fluorescent  
protein variants used for imaging studies in different species   

(It is possible to order the issue at a special price of 30 Euro, if a total  
of at least 50 orders is reached. Interested people, please, send a message  
to amueller@lrz.uni-muenchen.de). 

 
Submitted by: Salvo Bozzaro [salvatore.bozzaro@unito.it] 
============================================================================== 
[End dictyNews, volume 26, number 14]